Coding

Part:BBa_K3778002:Design

Designed by: Yun Tsai   Group: iGEM21_CCU_Taiwan   (2021-08-28)


Thrombin cleavage site for D2A21 mimic release (LVPRFA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acids glycine (G) and serine (S) in the thrombin cleavage site are respectively modified into phenylalanine (F) and alanine (A) to fit the first two amino acids of D2A21 (BBa_K3778007)

Source

Thrombin cleavage site for D2A21 mimic release is cloned and modified from the human F2 gene.


References